Implemented kill switches: 2
Kill Switch 1
Class
Repression of holin with multiple linearly arranged repressors
Toxins
| Name |
Number |
Comment |
| Holin |
|
|
| Lysozyme |
K112022 |
|
Topology
Parts
| Name |
Number |
Comment |
| arabinose |
|
|
| araC |
|
|
| IPTG |
|
|
| LacI |
|
|
| cI repressor |
|
|
| promoter |
R0051 |
|
| P.A.S.E 1 |
K1223001 |
|
Kill Switch 2
Class
Disfunctional essential protein in absense of fulfillment of parallel circuit
Antagonists
| Name |
Number |
Comment |
| Tyrosine |
|
|
Topology
Parts
| Name |
Number |
Comment |
| IPTG |
|
|
| LacI |
|
|
| lacI promoter |
|
|
| tRNA |
|
|
| tRNA synthetase |
|
|
| UAA (propargyl-L-lysine) |
|
|
| IPTG |
|
|
| lacI |
|
|
| lac/ara-L promoter |
K354000 |
|
| Tyr Synthetase (modified with TAG mutation) |
|
|
| two resistances for selection |
|
|